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For Hoecst staining, tissue was washed in PBS, blocked in 2% BSA, 0.1% Triton X-100, washed and stained using Hoecst (Thermo Fisher 1:2000)
The second hook is placed just behind the dorsal fin by scratching off a few scales, pushing the hook through, and securing it on the other side with another small piece of rubber pushed past the barb
Do circle hooks work on largemouth bass
Thank you, Chris 1/4oz to 1 3/4oz
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